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Fluorescein Isothiocyanate

Definition: Fluorescein isothiocyanate (FITC) is a fluorescent dye commonly used for labeling and tagging various biological molecules and cellular structures in scientific research and diagnostic applications.

Uses:

1. Biochemical Research:

In biochemical research, fluorescein isothiocyanate is often used as a conjugate to label proteins, antibodies, peptides, and nucleic acids for visualizing and studying their interactions within cells and tissues.

Example Sentence:

The fluorescein isothiocyanate conjugated to the antibody allowed us to visualize the localization of the target protein within the cell.

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2. Diagnostic Applications:

Fluorescein isothiocyanate finds application in diagnostic techniques such as immunofluorescence assays and flow cytometry to detect and characterize specific cells or biomarkers.

Example Sentence:

The fluorescence emitted by the fluorescein isothiocyanate-labeled cells enabled accurate identification and separation using flow cytometry.

Properties:

Fluorescein isothiocyanate exhibits high quantum yield and excellent photostability, making it a reliable and commonly used fluorescent probe in biological research.

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